蛋白表达服务

1.用于提高基因表达水平的OptimumGene^TM密码子优化技术

金斯瑞先进的OptimumGene^TM密码子优化技术能帮助您优化基因序列，使基因表达和蛋白溶解度达到较高水平。OptimumGene^TM技术还可用来优化很多与转录、翻译、蛋白折叠相关的参数。

2.较高的成功率以及丰富的重组蛋白制备经验

我们已经为客户制备了数以千记的复杂蛋白,其中包括：跨膜蛋白,分泌蛋白,蛋白酶以及其它的酶类,成功率均在90%以上。金斯瑞制备的重组蛋白已成功应用于靶点确认、酶鉴定、抗体制备、高通量筛（HTS）、 构象研究以及SAR（结构-活性关系）。

3.BacPower^TM技术和FoldArt^TM蛋白折叠技术

金斯瑞自主研发的BacPower^TM技术和FoldArt^TM蛋白折叠技术，使蛋白难表达和蛋白难溶等问题迎刃而解。

4.全面的服务

金斯瑞能为您提供基因合成，蛋白表达，稳定细胞株生产，抗体制备以及免疫检测等一体化服务。

5.高质量的服务

金斯瑞高质量的蛋白表达服务,可满足您对蛋白的纯度高、活性强、交付时间快等需求。金斯瑞交付的蛋白一般纯度在95%以上,内毒素水平低于0。01 EU/µg。

药物研发过程中，制备和纯化后的蛋白具有生物活性是十分必要的。金斯瑞先进的设备和专利的制备方法，保证了交付给客户的蛋白均具有生物活性。我们进行蛋白表达与免疫检测相结合，交付给客户的高质量、高活性蛋白，甚至超过了客户的预期，具体请参考下面的示例。我们通过对细胞破壁方法进行优化，提高了纯度大于90%的目标蛋白的获得量。

• Fig.A: SDS-PAGE analysis of the fractions upon the IEX chromatography 。
• Lane 1-47: Gradient elution fractions
• Lane M: Low weight marker (Jinsite, Cat. No. MM0906)
• Lane S: The supernatant after homogenizer with high pressure
• Lane ft: Flow through

• Fig.B: Enzyme assay monitoring the fractionation by DEAD Sepharose

• Fig. C: Fractions pooled based on activity analysis for further ADP Sepharose affinity purification
• Fig. D: SDS-PAGE analysis of the purified protein
• Lane M: Low weight marker (Jinsite, Cat. No. MM0906)
• Lane S: The supernatant after homogenizer with high pressure
• Lane ft: Flow through

金斯瑞专利的蛋白折叠技术，可以解决原核系统内由于过量表达而带来的蛋白活性丧失、折叠错误、蛋白发生集聚反应不能溶解等问题。我们可以成功溶解并折叠含有95%以上包涵体的目标蛋白（请参考下述示例），折叠蛋白的纯度高达85%以上。金斯瑞交付给客户的折叠蛋白储存于特制缓冲液中。

• Fig. Refolded protein of sample was analyzed by SDS-PAGE followed by Coomassie Brilliant Blue staining
• Lane M: Smart Protein Standard (Jinsite, Cat. No. MM0900)
• Lane 1: Refolded protein of sample

重组蛋白中如果含有表位附加标签，可能会导致蛋白结构改变、产生毒性，甚至丧失活性。为了避免这类问题，很多客户在蛋白表达后要求去除标签。在下面的示例中，金斯瑞使用SUMO蛋白酶，成功去除了标签。获得的目标蛋白标签去除率为80%，纯度高达90%以上。

SDS-PAGE checking purification of protein after tag cleavage by SUMO protease

Fig. A:

• Lane M: Smart Protein Standard (Jinsite, Cat.No. MM0900)
• Lane 1: Refolded fusion protein with SUMO-tag

Fig。 B:

• Lane M: Smart Protein Standard (Jinsite, Cat.No. MM0900)
• Lane 1: Recovered protein after 1st incubation with Ni-resin
• Lane 2: Recovered protein after 2nd incubation with Ni-resin
• Lane 3: Elute with 300 mM imidazole from Ni-resin of 2nd incubation

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